JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
考试流式细胞仪检测胸腺阳性和阴性选择
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Examination of Thymic Positive and Negative Selection by Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
考试流式细胞仪检测胸腺阳性和阴性选择
DOI:
10.3791/4269-v
•
14:29 min
•
October 08, 2012
•
Qian Hu*
1
,
Stephanie A. Nicol*
1
,
Alexander Y.W. Suen*
1
,
Troy A. Baldwin
1
Department of Medical Microbiology and Immunology
,
University of Alberta
Chapters
00:05
Title
01:16
Dissection
02:51
Cell Preparation
04:06
Staining Cells for Flow Cytometry
05:22
Results: Thymic Positive and Negative Selection by Flow Cytometry
13:37
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们提出了一种基于流式细胞仪的方法来研究T细胞发育<em在体内</em使用基因操纵的野生型小鼠的T细胞受体的转基因背景。
Tags
Thymic Positive Selection
Thymic Negative Selection
Flow Cytometry
T Cell Receptor (TCR)
Antigen Specificity
Self-antigens
T Cell Repertoire
Autoimmune Disorders
Immunodeficiency Disorders
Double Negative (DN) Thymocytes
Double Positive (DP) Stage
CD4 Co-receptor
CD8 Co-receptor
Self-peptide-MHC (pMHC)
High Affinity Interactions
Low Affinity Interactions
Positive Selection
Single Positive (SP) T Cells
Foreign Antigens
Polyclonal TCR Repertoire
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
在固定和活细胞,流式细胞仪测量钙蛋白酶活性
人类<em在体外承认免疫失衡的早期国家禁止作为筛检工具
相互作用的研究<em>金黄色葡萄球菌</em与中性粒细胞通过流式细胞仪和时间推移显微镜
检测荧光纳米粒子的相互作用与原发性免疫细胞亚群流式细胞仪的
在
啮齿动物
疟原虫
原虫的
体内
评估和裂殖子入侵流式细胞仪
通过流式细胞仪测量附件和流感的内在病毒在A549细胞
通过流式细胞仪大鼠肾脏巨噬细胞的表型特征
荧光激活细胞分选从小鼠骨髓浆树突状细胞的分离纯化
小鼠与人肺泡巨噬细胞的分离和
体外
培养
全血流式细胞术分析人单核细胞亚群的表征
Read Article