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Chapters
- 00:05Title
- 01:23Protein Expression and Purification
- 03:41DNA Purification
- 07:35Protein-DNA Complex Formation and Analysis
- 08:35Results: Crystal Structure of SeqA delta(41-59)-A25R Bound to Hemimethylated DNA
- 11:08Conclusion
Kristalstructuur van eiwit-DNA-complexen kan inzicht verschaffen in eiwitfunctie, mechanisme, evenals de aard van de specifieke interactie. Hier rapporteren we hoe de lengte en sequentie uiteinden van duplex DNA optimaliseren voor co-kristallisatie met<em> Escherichia coli</em> SeqA, een negatieve regulator van replicatie-initiatie.
Tags
DNA DuplexesCrystallizationSeqA-DNA ComplexesEscherichia Coli SeqANegative RegulatorDNA ReplicationPremature Reinitiation EventsHemimethylated GATC ClustersOrigin Of ReplicationReplication ForksHigher Ordered StructuresHigh Affinity ComplexesMinimal Functional UnitDimerGATC SequencesMolecular LevelStructural AnalysisProtein-DNA CrystallizationPacking InteractionsProtein-protein Interactions
