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Viral Tracing of Genetically Defined Neural Circuitry
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JoVE Journal Neuroscience
Viral Tracing of Genetically Defined Neural Circuitry

Viral Tracing of Genetically Defined Neural Circuitry

DOI:
10.3791/4253-v

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13:06 min

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October 17, 2012

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,
1Department of Genetics,Harvard Medical School, 2Howard Hughes Medical Institute,Harvard Medical School

Chapters

  • 00:05Title
  • 01:16Passage and Concentration of Vesicular Stomatitis Virus (VSV)
  • 04:23Stereotaxic Injection of Virus
  • 08:19Tissue Harvest and Preparation for Microscopic Analysis
  • 09:41Results: Behavior of rVSV(A/RG) Virus in Various Cell Types
  • 12:22Conclusion

Summary

Automatic Translation

Automatic Translation

A method of tracing synaptically connected neurons is described. We use TVA specificity of an upstream cell to probe whether a cell population of interest receives synaptic input from genetically defined cell types.

Tags

Viral TracingGenetically Defined Neural CircuitryTranssynaptic VirusesPseudorabies VirusRabies VirusVesicular Stomatitis VirusAnterograde DirectionRetrograde DirectionMonosynaptic Retrograde TracingGlycoprotein GeneChimeric GTVA ReceptorInputs Onto A Defined Cell TypeVSV As A Transsynaptic Tracer

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