JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
DNA損傷応答タンパク質の2次元および3次元生細胞イメージング
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
Please note that all translations are automatically generated.
Click here for the English version.
DNA損傷応答タンパク質の2次元および3次元生細胞イメージング
DOI:
10.3791/4251-v
•
10:24 min
•
September 28, 2012
•
Jason M. Beckta
2
,
Scott C. Henderson
,
Kristoffer Valerie
2,4
1
Department of Radiation Oncology
,
Virginia Commonwealth University
,
2
Department of Biochemistry & Molecular Biology
,
Virginia Commonwealth University
,
3
Department of Anatomy & Neurobiology
,
Virginia Commonwealth University
,
4
Massey Cancer Center
,
Virginia Commonwealth University
Chapters
00:05
Title
02:07
Cell Preparation
02:41
Microscope Setup and Image Acquisition
07:56
Image Processing and Analysis
09:02
Results: 53BP1 Foci Formation
09:41
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
このプロトコルは、DNA損傷だけでなく、有糸分裂時にその局在化に応答して活性化されたDNA二重鎖切断シグナル伝達タンパク質を可視化するための方法を説明します。
Tags
Two-dimensional
Three-dimensional
Live Cell Imaging
DNA Damage Response Proteins
Double-strand Breaks
Mutations
Chromosomal Aberrations
Genomic Instability
DNA Damage Response
Cell Cycle Arrest
Apoptosis
Nonhomologous End-joining
Homologous Recombination Repair
Proteins
53BP1
P53-binding Protein 1
Modified Histones
Foci
Histone Modifications
Chromatin Rearrangement
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
三次元細胞培養用チップサイズの足場の微細加工
ライブゼブラフィッシュの胚における二光子軸索切断とタイムラプス共焦点イメージング
のライブイメージング
キイロショウジョウバエ
胚性血球移行
イメージングのミスマッチ修復とのDNA損傷に対する細胞応答
枯草菌</em
COS - 7の蛍光標識は、生細胞イメージングのためにSNAP - Tag融合タンパク質を発現する
ライブゼブラフィッシュ胚における二光子ベースの光活性化
でGFP標識タンパク質のイメージングを生きる<em>ショウジョウバエ</em>卵母細胞
マウス胎児腸全体培養システム<em>生体外</em>シグナル伝達経路の操作と絨毛開発の3次元ライブイメージング
三次元ハイドロゲルでイメージングをFRET
裏打ちクリア: 組織の脂肪組織の三次元イメージング法をクリア
Read Article