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粒细胞相关的自身抗体引起的皮肤起泡
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Granulocyte-dependent Autoantibody-induced Skin Blistering
DOI:

12:23 min

October 12, 2012

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Chapters

  • 00:00Title
  • 01:28Overall Scheme of the Experiment
  • 02:36Preparation of Pathogenic Antibody
  • 04:06Injection of Collagen VII-specific IgG into Mice
  • 06:47Clinical Evaluation of Disease Severity
  • 09:13Analysis of Tissue-bound and Circulation Collagen-specific Antibodies
  • 10:19Representative Results
  • 11:41Conclusions

Summary

Automatic Translation

在动物模型中描述在我们目前的工作中,纯化的IgG抗体对拉伸的200个氨基酸(氨基酸757-967)的胶原纤维VII反复被注入到小鼠体内,再现起泡表型以及直方和免疫病理学特征对人体acquisita大疱性表皮松解症(EBA)<sup> 1</sup>。

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