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Journal
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Immunology and Infection
/
流感聚合酶PB2亚基基因结构测定多目标并行处理方法
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
Please note that all translations are automatically generated.
Click here for the English version.
流感聚合酶PB2亚基基因结构测定多目标并行处理方法
DOI:
10.3791/4225-v
•
22:10 min
•
June 28, 2013
•
Brianna L. Armour*
1
,
Steve R. Barnes*
2
,
Spencer O. Moen
,
Eric Smith
,
Amy C. Raymond
,
James W. Fairman
,
Lance J. Stewart
,
Bart L. Staker
,
Darren W. Begley
,
Thomas E. Edwards
,
Donald D. Lorimer
1
Protein Crystallization Lab
,
Emerald Bio
,
2
Molecular Biology Lab
,
Emerald Bio
,
3
Scientific Sales Representative
,
Emerald Bio
,
4
Group Leader II
,
Emerald Bio
,
5
Group Leader I
,
Emerald Bio
,
6
Chair of Advisory Board
,
Emerald Bio
,
7
Director of Multi-Target Services
,
Emerald Bio
,
8
Senior Project Leader
,
Emerald Bio
,
9
Project Leader II & SSGCID Site Manager
,
Emerald Bio
Chapters
00:05
Title
02:20
Construct Design
04:07
Polymerase Incomplete Primer Extension (PIPE) Cloning
06:14
Expression Optimization
08:21
Large Scale Fermentation
09:40
Protein Purification – Cell Lysis
10:54
Protein Purification – Affinity Chromatography
13:04
Protein Purification – Affinity Tag Cleavage
14:47
Protein Purification – Size-exclusion Chromatography (SEC)
15:42
Protein Crystallization
17:03
Crystal Harvesting
18:15
Crystal Screening/Data Collection
19:27
Results: 3D Structure Determination of Influenza Virus PB2
21:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
基于结构的药物设计在药物发展中起着重要的作用。并行追求多个目标,率先发现的成功的机会大大增加。下面的文章强调了西雅图结构基因组学中心传染病流感A亚基PB2基因到结构的确定采用了多目标的方法。
Tags
Multi-target
Parallel Processing
Gene-to-structure Determination
Influenza Polymerase PB2 Subunit
Pandemic Outbreaks
Influenza Virus Infection
Point Mutations
Virulence Factor
Antiviral Drug Target
Structural Genomics Program
National Institute Of Allergy And Infectious Disease (NIAID)
Emerald Bio
Seattle Structural Genomics Center For Infectious Disease (SSGCID)
Three-dimensional Protein Structures
NIAID Category A-C Pathogens
Structure-based Drug Design
Drug Development
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