Journal
/
Medicine
/
修复的临界大小的颅骨缺损模型脂肪间充质干细胞的收获Lipoaspirate
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate
Please note that all translations are automatically generated. Click here for the English version.

修复的临界大小的颅骨缺损模型脂肪间充质干细胞的收获Lipoaspirate

DOI:
10.3791/4221-v

•

11:31 min

•

October 31, 2012

•

, , , , , , , ,
1Department of Surgery,Stanford University , 2Department of Surgery,Duke University , 3Department of Surgery,Saint Joseph Mercy Hospital, 4School of Medicine,University of California, San Francisco , 5School of Dentistry,University of California, Los Angeles

Chapters

  • 00:05Title
  • 01:38Cell Isolation and Expansion
  • 05:10Cell Seeding, Creating Calvarial Defects and in vivo Implantation
  • 07:47Quantification of Osteoid Formation
  • 09:07Results: Repair of Critical-size Defect by Human Adipose-derived Stromal Cells
  • 10:50Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了隔离的脂肪组织的基质细胞从lipoaspirate 4毫米的临界大小的颅骨缺损，以评估骨骼的再生和创造的。

Tags

RepairCritical-sized Calvarial DefectAdipose-derived Stromal CellsLipoaspirateCraniofacial Skeletal RepairCell-based ApproachStem CellsOsteogenic PotentialScaffolding BiomaterialsCellular DeliveryOsteoconductiveHydroxyapatite-coated Poly(lactic-co-glycolic Acid)HA-PLGA ScaffoldOsseous RegenerationTranslational ApproachesProgenitor Cell

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

从人类乳腺成纤维细胞癌相关的成纤维细胞的实验发电（CAFS）

单侧颈脊髓损伤的挫伤模型，利用无限的地平线冲击

建立评估膜内骨化硬性稳定骨折，牵张成骨，或临界中小企业缺损愈合

一种新的小鼠模型主动脉瘤腔内修复

两种方法从子宫切除标本小学建立人子宫内膜基质细胞

对无瘢痕修复鼠标胎儿皮肤模型

一个简单的临界尺寸股骨缺损模型小鼠

在小鼠节段性股临界尺寸缺陷模型的建立接骨板稳定

创作和移植的糖尿病伤口愈合模型中脂肪源性干细胞 (ASC) 表

CoCrMo 颗粒暴露的小鼠颅骨骨溶解模型的构建与评价

Read Article