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体外评估对离体骨骼肌的收缩，疲劳性和交替“
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JoVE Journal Biology
Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles
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体外评估对离体骨骼肌的收缩，疲劳性和交替“

DOI:
10.3791/4198-v

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14:02 min

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November 01, 2012

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, , , , ,
1Department of Physiology and Biophysics,UMDNJ-Robert Wood Johnson Medical School, 2Muscle Biology Research Group,University of Missouri-Kansas City, 3Pharmacology division, College of Pharmacy, DHLRI,Ohio State University

Chapters

  • 00:05Title
  • 02:22Preparing Intact Muscle Bundles
  • 05:55Measuring Contractility of the Isolated Skeletal Muscles
  • 11:25Results: Ex Vivo Assessment of Contractility, Fatigability and Alternans in Murine Skeletal Muscles
  • 12:19Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一种方法来直接测量肌肉力量，肌肉力量，收缩动力学和离体骨骼肌疲劳性的<em在体外</em系统使用领域的刺激。有价值的信息，对Ca<sup> 2 +</sup>处理性和机械的肌肉收缩，可以使用不同的刺激协议获得。

Tags

Ex Vivo AssessmentContractilityFatigabilityAlternansIsolated Skeletal MusclesMuscle ForceMuscle PowerContractile KineticsRecovery After FatigueExcitation-contraction Coupling (ECC)ExcitabilityContractile MachineryCa2+ Handling AbilityGenetic ComponentsCa2+ Signaling PathwaysSkeletal Muscle PhenotypeIn Vitro Muscle Contractility AssaySingle Cell AssaysGenetic ApproachesBiochemistry AssaysMechanisms Of ECC

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