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培养成年的背根神经节神经元轴突再生的遗传研究

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

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培养成年的背根神经节神经元轴突再生的遗传研究

DOI:

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09:42 min

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August 17, 2012

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Saijilafu, Feng-Quan Zhou²

¹Department of Orthopaedic Surgery,Johns Hopkins University School of Medicine, ²Solomon H. Snyder Department of Neuroscience,Johns Hopkins University School of Medicine

Chapters

00:05Title
01:50Preparing Neuronal Culture Coverslips
02:37Collecting Adult Mouse DRG Neurons
04:12Preparing Adult Mouse DRG Neurons for Electroporation
05:40Electroporation and Culturing of Neurons
08:03Genetically Manipulated Adult Mouse DRG Cultures
09:15Conclusion

Summary

Automatic Translation

一个<em>在体外</em用培养的成年小鼠背根神经节神经元的轴突再生的遗传研究模型描述。方法包括re-suspension/re-plating的步骤，允许进行遗传操作的神经元的轴突重新生长。这种做法是轴突再生功能丧失使用RNAi为基础的蛋白质击倒的研究特别有用。

Tags

Genetic Study Axon Regeneration Cultured Adult Dorsal Root Ganglion Neurons Central Nervous System (CNS)Peripheral Nervous System (PNS)Mature Neurons Axon Growth Hostile Environment Peripheral Nerve Injuries Conditioning Lesion Spinal Cord Injury Molecular Mechanisms CNS Axon Regeneration

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