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小规模的核提取物,基因表达机具功能分析
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries
Please note that all translations are automatically generated.
Click here for the English version.
小规模的核提取物,基因表达机具功能分析
DOI:
10.3791/4140-v
•
08:05 min
•
June 27, 2012
•
Eric G. Folco
,
Haixin Lei
,
Jeanne L. Hsu
,
Robin Reed
1
Department of Cell Biology
,
Harvard Medical School
Chapters
00:05
Title
01:05
Growing and Harvesting HeLa Cells for Nuclear Extraction
01:56
Swell HeLa Cells in Hypotonic Buffer
03:00
Lyse Cells using a Dounce Homogenizer
04:27
Salt-extract the Nuclei
05:15
Concentrate and Dialyze the Nuclear Extract
06:16
Results: Small-scale Nuclear Extract Technique for Functional Assays of Gene Expression Machineries
07:51
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
一个健壮的,小规模的HeLa细胞的核提取物制备的协议。该协议是为需要使用小种群的细胞,如细胞治疗药物或RNAi,有价值的实验。该方法应该适用于各种各样的基因表达分析和其他类型的细胞,包括病人的细胞。
Tags
Small-scale Nuclear Extracts
Functional Assays
Gene Expression
In Vitro Systems
Bulk Preparations
In Vivo Cellular Treatments
Small-scale Protocol
RNA Polymerase II Transcription/splicing Reactions
Splicing Inhibitor Drug E7107
Limited Quantities
Small Cell Populations
Freshly Grown Cells
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