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病原液泡的分离纯化<em>军团</em>感染吞噬细胞
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Purification of Pathogen Vacuoles from Legionella-infected Phagocytes
DOI:

09:25 min

June 19, 2012

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Chapters

  • 00:05Title
  • 01:23Preparations for the Production of Legionella pneumophila-containing Vacuoles (LCVs)
  • 02:31LCV Isolation
  • 06:46Microscopic Analysis of the Collected Samples
  • 07:38Representative Results
  • 09:00Conclusion

Summary

Automatic Translation

本文介绍了一个完整的隔离和净化的方法<em>军团</em>从变形虫和巨噬细胞含有空泡(LCVs)。两步的协议,包括轻型商用车通过使用对细菌的轻型商用车标记和密度梯度离心进一步纯化抗体的免疫磁性分离富集。

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