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Journal
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Immunology and Infection
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朊病毒蛋白质错误折叠循环扩增
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Protein Misfolding Cyclic Amplification of Prions
Please note that all translations are automatically generated.
Click here for the English version.
朊病毒蛋白质错误折叠循环扩增
DOI:
10.3791/4075-v
•
10:12 min
•
November 07, 2012
•
Samuel E. Saunders
,
Jason C. Bartz
,
Ronald A. Shikiya
1
Department of Civil Engineering
,
University of Nebraska at Lincoln
,
2
Department of Medical Microbiology and Immunology
,
Creighton University
Chapters
00:05
Title
01:41
Preparing the Samples
03:13
Amplification of Prion Strains using PMCA
08:21
Results: Western Blot of PK-digested HaCWD and DY TME Hamster-derived Prion Strains
09:48
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
蛋白质错误折叠循环放大(PMCA)的的朊病毒转化和应变和物种障碍的研究是在体外试验。它也可以被用作朊病毒检测法。
Tags
Protein Misfolding
Cyclic Amplification
Prions
Transmissible Spongiform Encephalopathy
PrPC
PrPSc
Incubation Period
Clinical Signs
Animal Bioassay Studies
Biochemical Properties
Biophysical Properties
In Vitro Technique
Sonication
Incubation Cycles
PrPSc Seed
Species Specificity
Strain Specificity
Prion Strain Interference
Amplification
Fluids
Environmental Samples
PMCA Protocol
Contamination Control
Consistent Results
Quantification
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