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Neuroscience
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钙成像的急性下区片的制备
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Preparation of Acute Subventricular Zone Slices for Calcium Imaging
Please note that all translations are automatically generated.
Click here for the English version.
钙成像的急性下区片的制备
DOI:
10.3791/4071-v
•
09:10 min
•
September 19, 2012
•
Benjamin Lacar
,
Stephanie Z. Young
,
Jean-Claude Platel
,
Angélique Bordey
1
Department of Neurosurgery and Cellular & Molecular Physiology
,
Yale University School of Medicine
Chapters
00:05
Title
01:02
Removal of Brain Tissue
02:03
Acute Brain Slice Preparation
03:33
Preparation of the Microscope and Dyes for Imaging
04:50
Dye Loading: The Bath Method
05:46
Dye Loading: The Pressure Application Method
07:06
Confocal Imaging of Calcium Activity
08:02
Results: Calcium Activity in the SVZ
08:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
一个方法来加载脑室下区(SVZ)细胞中的钙离子指示剂染料记录活性钙。产后SVZ包括神经祖细胞和神经母细胞的细胞排列紧密。而不是使用清洗负载注入染料内的组织可以更好的染料扩散的压力。
Tags
Acute Subventricular Zone
Slices
Calcium Imaging
Neurogenic Zone
SVZ Astrocytes
Neuroblasts
Intermediate Progenitor Cells
Interneurons
Adhesion Molecules
Diffusible Signals
Intercellular Calcium Activity
Functional Intercellular Signaling
Calcium Indicator Dye
Bath Loading
Cellular Density
Sagittal Slices
Three-dimensional Arrangement
Neuroblast Migration
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