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一个从人体细胞分泌蛋白生产的方便,一般的表达平台
JoVE Journal
Biology
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JoVE Journal Biology
A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
DOI:

07:09 min

July 31, 2012

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Chapters

  • 00:05Title
  • 01:41Small-scale Test Expression
  • 03:34Large-scale Expression and Purification
  • 05:44Results: Human Cell Surface Proteins Purified from Large Scale HEK 293T Cells
  • 06:31Conclusion

Summary

Automatic Translation

在人类后基因组时代,在本机构的重组蛋白的结构,功能和治疗的研究和发展是至关重要的。在这里,我们描述了测试和大规模的蛋白质在人类胚胎肾细​​胞293T,可用于生产各种重组蛋白表达系统。

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