JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
分离人脐静脉内皮细胞和水流条件下其使用中的中性粒细胞轮回研究
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions
Please note that all translations are automatically generated.
Click here for the English version.
分离人脐静脉内皮细胞和水流条件下其使用中的中性粒细胞轮回研究
DOI:
10.3791/4032-v
•
08:54 min
•
August 08, 2012
•
Anutosh Ganguly
,
Hong Zhang
,
Ritu Sharma
,
Sean Parsons
,
Kamala D. Patel
1
Department of Physiology and Pharmacology
,
University of Calgary
Chapters
00:05
Title
01:41
Isolation and Maintenance of Human Vascular Endothelial Cells
04:54
Neutrophil Recruitment and Transmigration
06:36
Results: Fluorescent and Cell Migration Analysis of Neutrophil Transmigration Across Endothelial Cells in an Ibidi Chamber
08:03
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本文首先介绍了人类脐静脉内皮细胞的分离的过程,然后演示如何使用这些细胞检查流量条件下的中性粒细胞轮回。通过使用低体积流量室从玻璃的光学特性的聚合物制成,活细胞荧光成像罕见的细胞群也是可能的。
Tags
Isolation
Human Umbilical Vein Endothelial Cells
Neutrophil Transmigration
Flow Conditions
White Blood Cell
Innate Immune System
Acute Inflammation
Injury Site
Blood Vessels
Microvascular Structural Changes
Plasma Proteins
Rolling
Adhesion
Accumulation
In Vivo Methods
In Vitro Methods
Human Neutrophils
Primary Human Umbilical Vein Endothelial Cells (HUVEC)
Neutrophil Isolation
Confluence
Activation Molecules
Cytokines
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
量<em在体外</em>激活的主人微血管内皮细胞在静态条件下测量的检测,以中性粒细胞粘附
一个流程粘附实验来研究白细胞招聘剪切应力的人力肝窦内皮细胞条件下
人中性粒细胞流动室粘附实验
内皮细胞,细胞连接实时成像过程中中性粒细胞轮回在生理流动
在体外
和
体内
模型来研究细菌粘附于血管壁下流动条件
流式细胞仪用来评估染色质的T细胞国家
中性粒细胞分离和分析,以确定他们的淋巴瘤细胞的敏感性对治疗药物的作用
慢性炎症状态个体的单核细胞轮回和泡沫形成的定量研究
小鼠与人肺泡巨噬细胞的分离和
体外
培养
体外流动下单核细胞亚群的同时研究
Read Article