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核糖体束缚新生多肽的分离<em>在体外</em>确定沿mRNA的翻译暂停网站
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Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
Please note that all translations are automatically generated. Click here for the English version.

核糖体束缚新生多肽的分离<em>在体外</em>确定沿mRNA的翻译暂停网站

DOI:
10.3791/4026-v

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10:15 min

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July 06, 2012

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,
1Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences,Cleveland State University

Chapters

  • 00:05Title
  • 03:55DNA Template Preparation, In vitro Transcription, and In vitro Translation
  • 05:30Isolation and Resolution of Nascent Polypeptides
  • 06:54Representative Translational Pause Results
  • 08:35Conclusion

Summary

Automatic Translation

Automatic Translation

描述的一种技术，以确定mRNA的翻译暂停网站。本程序是基于对新生多肽在体外培养的靶mRNA的翻译，其次是使用变性凝胶电泳新生链的大小分析核糖体积累的隔离。

Tags

RibosomePolypeptidesTranslational Pause SitesMRNACodon UsageSynonymous CodonsNon-uniform ElongationMRNA Secondary StructureRibosome MovementTranslational Elongation RatesCodon BiasTRNAsRare CodonsPause SitesProtein ExpressionMRNA StabilityProtein Folding

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