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マウス胚性幹細胞の維持と自己複製を制御する遺伝子を研究するためにOct4GiPレポーターアッセイ
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JoVE Journal
Biology
Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
Please note that all translations are automatically generated.
Click here for the English version.
マウス胚性幹細胞の維持と自己複製を制御する遺伝子を研究するためにOct4GiPレポーターアッセイ
DOI:
10.3791/3987-v
•
08:01 min
•
May 30, 2012
•
Xiaofeng Zheng
,
Guang Hu
1
Laboratory of Molecular Carcinogenesis
,
National Institute of Environmental Health Sciences
Chapters
00:05
Title
01:38
siRNA Transfection in Oct4GiP Cells
04:05
FACS Analysis
06:07
Results: The Oct4GiP Reporter Assay Readily Detects the Differentiation Caused by Gene Silencing in the Maintenance of ES Cell Renewal
07:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我々はすぐに識別し、マウス胚性幹細胞の維持と自己複製を制御する遺伝子を特徴付けるための蛍光レポーターアッセイを説明します。
Tags
Oct4GiP
Reporter Assay
Genes
Regulate
Mouse Embryonic Stem Cell Maintenance
Self-renewal
Pluripotency
Molecular Mechanism
Developmental Biology Studies
Disease Modeling
Drug Discovery
Regenerative Medicine
Fluorescence Reporter-based Assay
Self-renewal Status
Mouse ES Cells
Oct4 Gene Promoter Region
Green Fluorescent Protein (GFP)
Differentiation
Fluorescence-activated Cell Sorting (FACS) Analysis
RNAi
Convenience
Sensitivity
Quantitative Analysis
Cost-effective
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