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Journal
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Neuroscience
/
大鼠胚胎神经细胞的分离和培养:一个快速议定书“
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol
Please note that all translations are automatically generated.
Click here for the English version.
大鼠胚胎神经细胞的分离和培养:一个快速议定书“
DOI:
10.3791/3965-v
•
10:51 min
•
May 24, 2012
•
Marco Pacifici
2
,
Francesca Peruzzi
2
1
LSU Health Sciences Center – New Orleans
,
2
Medical School and Stanley S. Scott Cancer Center
Chapters
00:05
Title
01:42
Poly-D-Lysine: Preparation and Coating of the Plastic Cell Culture Dishes
03:03
PDL and Laminin: Preparation and Coating of the Glass Two-chamber Slides
04:07
Neuronal Dissection and Culture
09:40
Results: Isolation and Culture of Rat Embryonic Neural Cells
10:27
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
我们描述了一个快速的方法分离和培养的海马和皮层神经细胞从老鼠胚胎。该协议允许在几乎纯神经细胞需要我们进行实验。
Tags
Isolation
Culture
Rat Embryonic Neural Cells
Quick Protocol
Dissociation
Hippocampal Neurons
Cortical Neurons
E15-17 Rat Embryos
Mouse Primary Neurons
Human Primary Neurons
Neural Progenitors
Serum-free Medium
Nucleofection
Immunocytochemistry
Nucleic Acids Preparation
Electrophysiology
Lentiviral Transduction
Calcium Phosphate Transfection
Lipid-based Transfection
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