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大鼠胚胎神经细胞的分离和培养:一个快速议定书“
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol
DOI:

10:51 min

May 24, 2012

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Chapters

  • 00:05Title
  • 01:42Poly-D-Lysine: Preparation and Coating of the Plastic Cell Culture Dishes
  • 03:03PDL and Laminin: Preparation and Coating of the Glass Two-chamber Slides
  • 04:07Neuronal Dissection and Culture
  • 09:40Results: Isolation and Culture of Rat Embryonic Neural Cells
  • 10:27Conclusion

Summary

Automatic Translation

我们描述了一个快速的方法分离和培养的海马和皮层神经细胞从老鼠胚胎。该协议允许在几乎纯神经细胞需要我们进行实验。

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