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液相亲和捕获试验用磁珠研究蛋白质相互作用:脊髓灰质炎病毒的Nanobody范例
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JoVE Journal
Biology
A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example
Please note that all translations are automatically generated.
Click here for the English version.
液相亲和捕获试验用磁珠研究蛋白质相互作用:脊髓灰质炎病毒的Nanobody范例
DOI:
10.3791/3937-v
•
08:10 min
•
May 29, 2012
•
Lise Schotte
,
Bart Rombaut
,
Bert Thys
1
Department of Pharmaceutical Biotechnology and Molecular Biology, Centre for Pharmaceutical Research
,
Vrije Universiteit Brussel
Chapters
00:05
Title
02:27
Preparation of the Magnetic Beads
03:00
Affinity Capture Assay
04:18
Recycling the Beads
05:44
Interpretation of the Results
06:13
Results: Liquid Phase Affinity Capture Assay using PVSS38C Nanobody
07:36
Conclusion
Summary
Automatic Translation
English (Original)
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在这篇文章中,提出了一个简单,定量,液相亲和力捕获检测。这是一个可靠的技术基础上,一方面与亲和力之间的标记蛋白,对其他的第二个标记蛋白(如脊髓灰质炎病毒)之间的磁珠和标签蛋白(如纳米抗体)的相互作用。
Tags
Liquid Phase Affinity Capture Assay
Magnetic Beads
Protein-protein Interaction
Tagged Protein
Labeled Protein
Cobalt Coated Magnetic Beads
Recognition Of The Tag
Bound Fraction
Unbound Fraction
Conformational Conversion Sensitive Proteins
Poliovirus-nanobody Interaction
ELISA
Solid Surface Attachment
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