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Bioluminescentie beeldvorming van NADPH-oxidase activiteit in verschillende diermodellen
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Chapters
- 00:05Title
- 02:09Animals Models: Intratracheal Administration of Zymosan
- 04:07Animal Models: Cecal Ligation and Puncture (CLP)
- 05:15Animal Models: Administration of Oral Carbon Tetrachloride
- 05:52Acquiring Bioluminescent Images
- 06:55Data Analysis using Region of Interest
- 07:45Detection of NADPH Oxidase Mediated ROS Production
- 09:57Conclusion
NADPH oxidase is de belangrijkste bron van reactieve zuurstof species (ROS) in fagocyten. Vanwege de vluchtigheid van ROS is moeilijk te meten en te controleren ROS levels in levende dieren. Een minimaal invasieve methode voor seriële kwantificering van ROS in levende muizen beschreven.
Tags
Bioluminescence ImagingNADPH Oxidase ActivityAnimal ModelsAntibacterial DefenseAntifungal DefenseInflammatory ResponseReal-time KineticsROS GenerationResearch ToolHost DefenseInflammationInjuryChronic Granulomatous DiseaseCGDInherited DisorderNADPH Oxidase ComponentsGp91phox-deficient MiceP47phox-deficient MiceImpaired Host DefensePhagocytesIsoforms Of NADPH OxidaseQuantify ROS ProductionModels Of Inflammation And Injury
