Journal
/
/
慢分裂的细胞在人脑胶质瘤羧基荧光素琥珀酰亚胺酯(CFSE)的分离与鉴定
JoVE Journal
Medicine
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Identification and Isolation of Slow-Dividing Cells in Human Glioblastoma Using Carboxy Fluorescein Succinimidyl Ester (CFSE)
DOI:

11:54 min

April 29, 2012

, , , ,

Chapters

  • 00:02Title
  • 00:44Introduction
  • 01:43Preparing Glioblastoma Single Cell Suspension
  • 03:06Staining with Cell Trace Carboxyfluorescein Succinimidyl Ester (CFSE) Green Fluorescent Dye
  • 05:37Flow Cytometry Analysis of CFSE Stained Glioblastoma Cells
  • 06:44Isolation of the Slow-dividing Cell Population using Fluorescent Activated Cell Sorting (FACS)
  • 10:02Representative Results
  • 11:12Conclusion

Summary

Automatic Translation

此视频协议演示的荧光染料羧基荧光素琥珀酰亚胺基酯(CFSE)的应用程序的不同的子种群的细胞分裂的频率的基础上在人成胶质细胞瘤细胞的识别和分离。

Related Videos

Read Article