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慢分裂的细胞在人脑胶质瘤羧基荧光素琥珀酰亚胺酯(CFSE)的分离与鉴定
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Identification and Isolation of Slow-Dividing Cells in Human Glioblastoma Using Carboxy Fluorescein Succinimidyl Ester (CFSE)
Please note that all translations are automatically generated.
Click here for the English version.
慢分裂的细胞在人脑胶质瘤羧基荧光素琥珀酰亚胺酯(CFSE)的分离与鉴定
DOI:
10.3791/3918-v
•
11:54 min
•
April 29, 2012
•
Loic P. Deleyrolle
,
Mark R. Rohaus
,
Jeff M. Fortin
,
Brent A. Reynolds
,
Hassan Azari
2
1
Department of Neurosurgery
,
The University of Florida
,
2
Department of Anatomical Sciences
,
Shiraz University of Medical Sciences, Shiraz, Iran
Chapters
00:02
Title
00:44
Introduction
01:43
Preparing Glioblastoma Single Cell Suspension
03:06
Staining with Cell Trace Carboxyfluorescein Succinimidyl Ester (CFSE) Green Fluorescent Dye
05:37
Flow Cytometry Analysis of CFSE Stained Glioblastoma Cells
06:44
Isolation of the Slow-dividing Cell Population using Fluorescent Activated Cell Sorting (FACS)
10:02
Representative Results
11:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
此视频协议演示的荧光染料羧基荧光素琥珀酰亚胺基酯(CFSE)的应用程序的不同的子种群的细胞分裂的频率的基础上在人成胶质细胞瘤细胞的识别和分离。
Tags
Slow-dividing Cells
Identification
Isolation
Human Glioblastoma
Carboxy Fluorescein Succinimidyl Ester (CFSE)
Tumor Heterogeneity
Therapeutic Strategies
Phenotypic Identification
Genetic Identification
Functional Identification
Tumor Subpopulations
Disease Pathologies
Clinically Relevant Targets
Cell Division Frequency
Brain Cancer
Breast Cancer
Skin Cancer
Pancreas Cancer
Leukemia
Fluorescent Dye CFSE
Tracking Cell Division Frequency
Blood-borne Tumors
Solid Tumors
Glioblastoma
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