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使用“单个病毒基因组的单病毒颗粒的分离和基因组分析'
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and Genome Analysis of Single Virions using ‘Single Virus Genomics’
DOI:

08:31 min

May 26, 2013

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Chapters

  • 00:05Title
  • 02:11Flow Cytometry
  • 03:54Visualizing Single Virions using Confocal Microscopy and Whole Genome Amplification
  • 06:34Results: Representative Isolation and Amplification of a Single Viral Genome
  • 07:54Conclusion

Summary

Automatic Translation

单一的病毒基因组(SVG)是一种方法来隔离和放大的基因组单virons。病毒悬浮液的混合组合,用流式细胞仪检测到载玻片离散井含琼脂糖,从而捕获病毒粒子和减少下游加工过程中的基因组剪切排序。全基因组扩增是通过使用多重置换扩增(MDA)的产生,是适合用于测序的基因组物质中。

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