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ECIS的/出租车细胞趋化测量
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Measurement of Cellular Chemotaxis with ECIS/Taxis
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ECIS的/出租车细胞趋化测量
DOI:
10.3791/3840-v
•
11:37 min
•
April 01, 2012
•
Kathryn M. Pietrosimone
,
Xiuyin Yin
,
David A. Knecht
,
Michael A. Lynes
1
Molecular and Cell Biology
,
University of Connecticut
,
2
University of Connecticut
Chapters
00:05
Title
01:28
ECIS/Taxis Electrode Preparation
03:13
Preparing Agarose Chambers and the Cannula Well-cutting Tool
04:21
Cutting Wells into Agarose
05:51
Loading the Wells
07:04
Data Collection
08:55
Data Management
10:13
Results: Chemotaxis of Human Jurkat T cells in Response to SDF1alpha
11:14
Conclusion
Summary
Automatic Translation
English (Original)
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企业家信心指数/出租车系统是一个自动化的,实时的检测,测量细胞趋化。在这个实验中,细胞的琼脂糖层到达目标电极下方移动。细胞运动耐交流电流0开始测量。
Tags
Cellular Chemotaxis
ECIS
TaxisCellular
Boyden Chamber Assay
Under-agarose Chemotaxis Assay
Chemoattractant Gradient
Solid Substrate
Aqueous Film
Agarose Layer
Automated ECIS/Taxis System
Electric Cell-substrate Impedance Sensing
Target Electrodes
Counter-electrode
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