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Journal
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Immunology and Infection
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遗传筛选隔离
弓形虫
宿主细胞的出口突变
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
A Genetic Screen to Isolate
Toxoplasma gondii
Host-cell Egress Mutants
Please note that all translations are automatically generated.
Click here for the English version.
遗传筛选隔离
弓形虫
宿主细胞的出口突变
DOI:
10.3791/3807-v
•
14:56 min
•
February 08, 2012
•
Bradley I. Coleman
,
Marc-Jan Gubbels
1
Department of Biology
,
Boston College
Chapters
00:05
Title
01:39
Titration of Mutagen
05:59
Enrichment of Egress Mutants
08:48
Validation of Mutant Phenotypes by Egress Assays
10:21
Cloning of Egress Mutants by Limiting Dilution
12:41
Representative Data
14:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
正向遗传学是一个功能强大的方法来解开如何在分子水平<em>弓形虫</em从它的宿主细胞外出。协议提供化学诱变寄生虫,丰富与缺陷突变引起的出口,并验证克隆突变体的表型。
Tags
Genetic Screen
Toxoplasma Gondii
Host-cell Egress Mutants
Obligate Intracellular Parasite
Lytic Replication Cycle
Active Invasion
Replication Inside Host Cell
Active Egress
Molecular Level
Signaling Pathways
Pharmacological Agents
Triggers Of Egress
Intracellular Ca2+
Candidate Gene Approach
Plant Like Calcium Dependent Protein Kinase (CDPK)
(chemical) Genetic Approaches
Screen For Mutant Parasites
Defect In Host Cell Egress
Chemical Mutagenesis
N-ethyl-N-nitrosourea (ENU)
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