A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:28Infecting Cells with Retrovirus Expression Reprogramming Factors
- 03:31Isolation and Expansion of Induced Pluripotent Stem Cells
- 04:42Analysis of Pluripotent Markers by Immunofluorescence and Quantitative Real-time PCR
- 06:06Representative Cell-reprogramming Results
- 08:03Conclusion
Une méthode pour générer de l'homme cellules souches pluripotentes induites (CISP) via un rétrovirus médiation expression ectopique de OCT4, SOX2, KLF4 et MYC est décrite. Un moyen pratique pour identifier l'homme colonies iPSC fondées sur l'expression de GFP est également discuté.
Tags
ReprogrammingHuman Somatic CellsInduced Pluripotent Stem CellsRetroviral VectorGFPHuman Embryonic Stem CellsPluripotentIn Vitro Disease ModelingRegenerative MedicineTranscription FactorsOct4Sox2Klf4MycAutologous CellsFibroblast CellsGFP-containing Retroviral BackboneESC Culture ConditionMorphologyRetroviral Transgene SilencingFluorescence MicroscopePluripotency GenesSurface Markers
