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共培养内皮细胞介导成熟的人类胚胎干细胞向胰腺中胰岛素生成细胞在定向分化的方法
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Biology
Endothelial Cell Co-culture Mediates Maturation of Human Embryonic Stem Cell to Pancreatic Insulin Producing Cells in a Directed Differentiation Approach
Please note that all translations are automatically generated.
Click here for the English version.
共培养内皮细胞介导成熟的人类胚胎干细胞向胰腺中胰岛素生成细胞在定向分化的方法
DOI:
10.3791/3759-v
•
08:29 min
•
March 27, 2012
•
Maria Jaramillo
,
Ipsita Banerjee
2
1
Department of Bioengineering
,
University of Pittsburgh
,
2
Department of Chemical Engineering
,
University of Pittsburgh
Chapters
00:05
Title
01:20
Culturing Human Embryonic Stem Cells
02:05
Definitive Endoderm and Pancreatic Progenitor Induction
03:01
Pancreatic Maturation
03:57
qRT-PCR Analysis
05:06
Immunocytochemistry
06:21
Analysis of Differentiation of Human Embryonic Stem Cells into Insulin-producing Cells
08:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
目前的研究,介绍了胰腺人类胚胎干细胞分化诱导定向分化的方法。具有重要意义的发现,血管内皮细胞共培养介导派生为胰岛素表达细胞的胰腺祖细胞的人类胚胎干细胞的成熟。
Tags
Endothelial Cell Co-culture
Human Embryonic Stem Cells
Directed Differentiation Approach
Pancreatic Insulin Producing Cells
Embryonic Stem Cells
Pluripotent
Cell Based Therapy
Regenerative Treatment
Cellular Differentiation
Organogenesis
Pancreas Development
Endoderm Induction
Nodal Pathway Modulation
Activin A
Growth Factors
Sonic Hedgehog Inhibition
Cyclopamine
Pancreatic Maturation
Notch Signaling Inhibition
Vascularization
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