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小鼠多巴胺和三维胶原基质检测纹状体外植体的解剖和文化
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Dissection and Culture of Mouse Dopaminergic and Striatal Explants in Three-Dimensional Collagen Matrix Assays
Please note that all translations are automatically generated.
Click here for the English version.
小鼠多巴胺和三维胶原基质检测纹状体外植体的解剖和文化
DOI:
10.3791/3691-v
•
08:24 min
•
March 23, 2012
•
Ewoud R.E. Schmidt
,
Francesca Morello
,
R. Jeroen Pasterkamp
1
Department of Neuroscience & Pharmacology, Rudolf Magnus Institute for Neuroscience
,
University Medical Center Utrecht
Chapters
00:05
Title
01:23
Dissection of the Dopaminergic Midbrain
03:03
Dissection of the Striatum
04:04
Assembly Collagen Matrix Assays
05:24
Analysis by Immunohistochemistry and Quantification
07:27
Results: Axon Growth in a Collagen Matrix Culture
08:00
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
中脑多巴胺系统及纹状体植为胶原基质检测<em>在体外</em>分析的mesostriatal和纹状体黑通路发展。轴突生长和指导,在此法可以操纵和量化。它也可以被修改为评估其他地区或分子线索。
Tags
Dissection
Culture
Mouse
Dopaminergic
Striatal
Explants
Three-dimensional Collagen Matrix Assays
Midbrain Dopamine Neurons
Striatum
Axon Tracts
Axon Growth
Guidance Cues
Soluble Factors
In Vitro
Collagen Matrix
Stable Gradients
Proteins Released
Rat Tail Collagen Purification
Microdissection
Dopaminergic Explants
Striatal Explants
Collagen Gels
Immunohistochemical Analysis
Quantitative Analysis
Axonal Projections
Article
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