JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
确定最佳人力Her2neu特异性CD8 + T细胞的细胞毒活性的比较CR51发行含量xCELLigence系统
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System
Please note that all translations are automatically generated.
Click here for the English version.
确定最佳人力Her2neu特异性CD8 + T细胞的细胞毒活性的比较CR51发行含量xCELLigence系统
DOI:
10.3791/3683-v
•
11:31 min
•
August 08, 2012
•
Courtney L. Erskine
,
Andrea M. Henle
,
Keith L. Knutson
1
Department of Immunology
,
College of Medicine, Mayo Clinic
Chapters
00:05
Title
01:16
Generation of Short-term Human Antigen-specific CD8 T Cell Lines
02:55
Preparation of Breast Cancer Cells
04:19
Co-culture of Tumor with CD8 T Cells
05:45
Chromium Release Assay (CRA)
07:21
HER-2/neu p369-specific T Cell / SKBR3 Breast Cancer Cells Co-cultures
11:09
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
铬释放法检测细胞毒性T细胞的活性,常见的检测,有几个限制。阻抗为基础的方法,在本文章中使用抗原特异性CD8 + T细胞和人乳腺癌肿瘤细胞系,SKBR3,检验检测细胞的杀伤能力。
Tags
Cytotoxic Activity
CD8 T Cells
Cr51 Release Assay
XCELLigence System
T Cell Receptor (TCR)
Antigen-specific CD8 T Cells
ELIspot
Flow Cytometry
Cytotoxicity Assay
Chromium Release Assay (CRA)
Gamma Radiation
Reproducibility
Caspase Release
BLT Esterase Activity
CD107 Expression
Impedance-based Assay
Roche XCelligence System
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
β细胞死亡介导细胞毒性T淋巴细胞的方法来评估
确定临床抗体样品的吞噬活性
在体外试验
,以评估免疫调节途径对HIV特异性CD4 + T细胞效应功能的影响
基于细胞的流式细胞仪检测,以测量细胞毒活性
评估的HIV-1感染的CD4的先天传感<sup> +</sup>性T细胞浆细胞样树突细胞使用<em>体外</em>共培养体系。
从外周血人类同种异体抗原特异性T细胞的生成
巨细胞病毒特异性的自动化细胞富集的T细胞用于使用细胞因子捕获系统的临床应用
干细胞衍生的抗原特异性调节性T细胞对自身免疫的发展
猴免疫缺陷病毒特异性CD8分析<sup> +</sup> T细胞猕猴由肽MHC-I四聚体染色
原位
MHC-聚丙烯染色和定量分析确定抗原特异性 CD8 T 细胞在组织中的位置、丰度和表型
Read Article