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样品制备<em>结核分枝杆菌</em提取物对核磁共振的代谢组学研究
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Immunology and Infection
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JoVE Journal Immunology and Infection
Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies
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样品制备<em>结核分枝杆菌</em提取物对核磁共振的代谢组学研究

DOI:
10.3791/3673-v

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07:56 min

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September 03, 2012

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, , , ,
1School of Veterinary Medicine and Biomedical Sciences,University of Nebraska-Lincoln, 2Department of Chemistry,University of Nebraska-Lincoln

Chapters

  • 00:05Title
  • 01:41Culturing M. tuberculosis (Class III Agent)
  • 04:44Adapting the NMR Methodology
  • 06:36Results: M. tuberculosis Wild Type Metabolic Pool
  • 07:16Conclusion

Summary

Automatic Translation

Automatic Translation

代谢组学的档案<em>结核分枝杆菌</em>确定后在肉汤培养物的生长。条件可以改变，以测试的营养补充剂，氧化剂，抗结核剂的影响，对这种微生物的代谢的档案。提取物制剂的程序是适用于两个1D<sup> 1</sup> H和2D<sup> 1</sup> H-<sup> 13</sup> C NMR分析。

Tags

Mycobacterium TuberculosisNuclear Magnetic ResonanceMetabolomic StudiesSample PreparationDrug-resistant StrainsGene FunctionGene Expression AnalysisGlobal PatternsMetabolic CompositionSmall Molecular Weight ChemicalsTarget IdentificationAnti-tubercular ActivityNMR Technology

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