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从小学的人角质形成细胞器官筏文化的代
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Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Organotypic Raft Cultures from Primary Human Keratinocytes
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从小学的人角质形成细胞器官筏文化的代

DOI:
10.3791/3668-v

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07:26 min

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February 22, 2012

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1Department of Microbiology & Immunology,University of North Carolina-Chapel Hill, 2Lineberger Cancer Center,University of North Carolina-Chapel Hill

Chapters

  • 00:05Title
  • 01:02Preparation of Metal Raft Grids and Collagen Gels
  • 03:03Preparing Keratinocytes for Differentiation
  • 04:16Making the Raft Cultures
  • 05:39Representative Organotypic Raft Culture Experiments
  • 06:55Conclusion

Summary

Automatic Translation

Automatic Translation

一个<em>在体外</em>方法来模仿<em>在体内</em>上皮细胞分化的描述。许多病毒针对病毒生命周期的一部分上皮细胞，这种方法提供了一种研究病毒的手段：宿主的相互作用，更接近于，<em>在体内</em>。这种技术可用于小学的角质细胞，建立细胞系，以及正常或病变的活检组织。

Tags

Organotypic Raft CulturesPrimary Human KeratinocytesIn Vitro SystemEpithelial DifferentiationThree-dimensional CulturesKeratinocytesHuman Papillomavirus (HPV)Virus ProductionDysplastic LesionsEpithelial Cell CancersDrugs On Epithelial Cell DifferentiationAsselineau And PrunierasKopan Et Al.Collagen PlugsAir-liquid InterfaceStratify And DifferentiateFull Thickness EpitheliumDifferentiation-specific CytokeratinsHistological ExaminationMolecular TechniquesBiochemical Techniques

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