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Neuroscience
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胎児マウスから海馬ニューロンの単離および培養
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Neuroscience
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JoVE Journal
Neuroscience
Isolation and Culture of Hippocampal Neurons from Prenatal Mice
Please note that all translations are automatically generated.
Click here for the English version.
胎児マウスから海馬ニューロンの単離および培養
DOI:
10.3791/3634-v
•
10:27 min
•
July 26, 2012
•
Michael L. Seibenhener
,
Marie W. Wooten
1
Department of Biological Sciences
,
Auburn University
Chapters
00:05
Title
01:12
Set-up Prior to Harvest
02:09
Tissue Harvest
04:03
Tissue Dissociation
05:23
Neuron Trituration
06:42
Cell Plating
08:43
Immunofluorescence Results with Hippocampal Neuronal Cultures
09:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
私達は非常にフィーダーグリア細胞層を使用せずに出生マウスの脳から海馬神経細胞の精製培養のためのプロトコルを提供しています。
Tags
Isolation
Culture
Hippocampal Neurons
Prenatal Mice
Primary Cultures
Rat
Murine
Neurobiology
Cellular Mechanisms
Cellular Trafficking
Cellular Structure
Protein Localization
Biochemical Techniques
Memory
Learning
Contamination
Brain Cell Types
Specific Media
Neuron Growth
Dissection Techniques
Embryonic Day 19 (E19)
Mouse Pups
Trypsin
Mechanical Disruption
Pipettes
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