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Journal
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Immunology and Infection
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扩大目标,脐血细胞毒性T淋巴细胞,巨细胞病毒,EB病毒,腺病毒
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus
Please note that all translations are automatically generated.
Click here for the English version.
扩大目标,脐血细胞毒性T淋巴细胞,巨细胞病毒,EB病毒,腺病毒
DOI:
10.3791/3627-v
•
11:18 min
•
May 07, 2012
•
Patrick J. Hanley
,
Sharon Lam
2
,
Elizabeth J. Shpall
,
Catherine M. Bollard
2,4,5
1
Center for Cell and Gene Therapy
,
Baylor College of Medicine
,
2
Pathology and Immunology
,
Baylor College of Medicine
,
3
Department of Stem Cell Transplantation and Cellular Therapy
,
University of Texas M.D. Anderson Cancer Center
,
4
Medicine
,
Baylor College of Medicine
,
5
Department of Pediatrics
,
Baylor College of Medicine
Chapters
00:05
Title
02:14
Mononuclear Cell Isolation
03:53
Dendritic Cell (DC) Generation
05:35
Generation of EBV-LCL
06:20
CTL Initiation
09:22
Representative Results
10:34
Conclusions
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述的第一个好生产质量管理规范(GMP)标准的方法从脐带血幼稚T细胞的来源主要是病毒特异性细胞毒性T淋巴细胞(CTL)。
Tags
Cytotoxic T Lymphocytes
Umbilical Cord Blood
Cytomegalovirus
Epstein-Barr Virus
Adenovirus
Stem Cell Transplantation
Cord Blood Transplantation
Virus-specific CTL
Dendritic Cells
Adenoviral Vector
CMV Antigen Pp65
IL-7
IL-12
IL-15
EBV-transformed B Cells
EBV-LCL
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