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从查明的神经元和取食行为的钙信号的同时记录
果蝇</em
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Simultaneous Recording of Calcium Signals from Identified Neurons and Feeding Behavior of
Drosophila melanogaster
Please note that all translations are automatically generated.
Click here for the English version.
从查明的神经元和取食行为的钙信号的同时记录
果蝇</em
DOI:
10.3791/3625-v
•
06:55 min
•
April 26, 2012
•
Motojiro Yoshihara
1
Department of Neurobiology
,
University of Massachusetts Medical School
Chapters
00:05
Title
01:30
Constructing the Fly Brain Live Imaging and Electrophysiology Stage or “FLIES”
02:00
Preparing the Fly for Observation
04:41
Stimulating the Proboscis
05:28
Simultaneous Recording of the Proboscis Extension Reflex and Calcium Imaging
06:22
Conclusion
Summary
Automatic Translation
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Automatic Translation
果蝇,<em>果蝇</em>,延长喂养它的长鼻,从长鼻或睑板糖刺激。我有长鼻延伸(PER)的响应与钙成像技术相结合的意见,使我们能够监测活动在大脑中的神经元,同时与行为观察。
Tags
Simultaneous Recording
Calcium Signals
Identified Neurons
Feeding Behavior
Drosophila Melanogaster
Neuronal Activity
Behavior Analysis
Genetically Encoded Calcium Indicators
GCaMP1
Optogenetic Techniques
Thermogenetic Techniques
Activating Identified Neurons
Critical Neurons For Feeding Behavior
Proboscis Extension Response
PER
Sweet Stimulus
Sensory Cells
Suboesophageal Ganglion
SOG
Behavioral Observation
Experimental System
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