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组织芯片，免疫组织化学染色和数字化生产在人类蛋白质图集
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas
Please note that all translations are automatically generated.
Click here for the English version.
组织芯片，免疫组织化学染色和数字化生产在人类蛋白质图集
DOI:
10.3791/3620-v
•
09:08 min
•
May 31, 2012
•
Caroline Kampf
,
IngMarie Olsson
,
Urban Ryberg
,
Evelina Sjöstedt
,
Fredrik Pontén
1
Department of Immunology, Genetics and Pathology, Science for Life Laboratory
,
Uppsala University
Chapters
00:05
Title
01:38
Manual Tissue Microarray Production
05:59
How to Search for a Protein of Interest in the Human Protein Atlas
07:06
Representative Tissue Microarray Results
08:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
组织芯片允许一个有效的方法，从众多的组织中获得的并发信息。组织的代表零件组装成一个单一的石蜡块。从块中的部分用于免疫组化和蛋白表达模式的分析。数字扫描生成相应的图像数据分布。
Tags
Immunohistochemistry Staining
Digitalization
Human Protein Atlas
High-throughput Analysis
Protein Expression Patterns
TMA Block
Cores
Immunostaining Protocol
Experimental Variability
Patient Cohorts
Consecutive Sections
Antibodies
Protein Profiles
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