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Diseksiyon ve Chick Statoacoustic Ganglion ve Kollajen Jeller Omurilik eksplantlar Kültür Neurite akıbet Tahliller
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Chapters
- 01:10Bead Preparation
- 01:52Statoacoustic Ganglion (SAG) Dissection
- 03:20Spinal Cord Dissection
- 05:28The Explant Culture with Either Purified Proteins or Protein-coated Beads
- 07:53Visualizing of Neurite Outgrowth by Immunohistochemistry
- 09:02Representative Results from Neurite Outgrowth Assays
- 10:42Conclusion
Biz civciv E4 statoacoustic ganglion ve E6 omurilik eksplantlar incelemek ve kültürüne nasıl gösterilmektedir. Eksplantlarındaki 24 saat için 3D kollajen jel serum serbest koşullar altında kültüre. Neurite yanıt büyüme faktörü desteği orta ve protein kaplı boncuklar ile test edilir.
Tags
DissectionCultureChick Statoacoustic GanglionSpinal Cord ExplantsCollagen GelsNeurite Outgrowth AssaysSensory OrgansInner EarSAG NeuronsAxon Guidance CuesSurvival FactorsOtic Axons MisroutingGrowth FactorsNeurotrophin-3 (NT-3)Brain-derived Neurotrophic Factor (BDNF)Transgenic AnimalsIn Vitro MethodResponsiveness Of SAG NeuritesSoluble ProteinsMorphogensNeuron SurvivalEmbryonic Day 4 (E4)Three-dimensional Collagen Gels
