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Biology
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细胞内复性检测
JoVE Journal
Biology
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JoVE Journal
Biology
Intracellular Refolding Assay
Please note that all translations are automatically generated.
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细胞内复性检测
DOI:
10.3791/3540-v
•
07:18 min
•
January 24, 2012
•
Tamara Vanessa Walther
,
Danilo Maddalo
1
Institute of Toxicology and Genetics
,
Karlsruhe Institute of Technology
Chapters
00:05
Title
01:12
Seeding the Cells
02:08
Transfection
03:02
Heat Shock
03:47
Cell Lysis and Luciferase Assay
06:17
Protein Refolding Dynamics
06:52
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这个协议的方法来衡量热休克后细胞内蛋白质复性的描述。这种方法可以用来研究如分子伴侣和他们的共同因素或化合物能够影响他们的活动foldases。萤火虫荧光素酶的活动是作为记者来衡量伴侣复性活动。
Tags
Intracellular Refolding Assay
Enzymatic Activity
Molecular Chaperones
Compounds
Inhibitory Activity
Stimulating Activity
Cell-based System
Cell Survival
Stress Insults
Heat Shock
Nutrient Starvation
Chemicals
Poisons
Tumor Development
Chemioresistance Of Cancer Cells
Neurodegeneration
Small Molecules
Cancer Therapy
Neurodegenerative Disorders
Refolding Activity
Gene Transfection
Firefly Luciferase Gene
Renilla Luciferase Gene
Protein Synthesis Inhibition
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