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Biology
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免疫染色的解剖斑马鱼胚胎心脏
JoVE Journal
Biology
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JoVE Journal
Biology
Immunostaining of Dissected Zebrafish Embryonic Heart
Please note that all translations are automatically generated.
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免疫染色的解剖斑马鱼胚胎心脏
DOI:
10.3791/3510-v
•
06:23 min
•
January 10, 2012
•
Jingchun Yang
,
Xiaolei Xu
1
Division of Cardiovascular Diseases, Department of Biochemistry and Molecular Biology
,
Mayo Clinic College of Medicine
Chapters
00:05
Title
01:08
Preparation of Slides and Humidified Chamber
01:50
Dissection of Embryonic Heart
03:21
Immunostaining and Imaging
05:00
Representative Heart Imaging Results
05:57
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
一个快速的方式来进行免疫的斑马鱼胚胎心脏描述。整装免疫方法相比,这种方法大大增加渗透的抗体,这使得获取高分辨率图像,揭示了心脏细胞/亚细胞结构内大幅减少处理时间。
Tags
Immunostaining
Dissected
Zebrafish
Embryonic Heart
In Vivo Model
Cardiac Development
Heart Diseases
Embryos
Transparent Embryos
Cardiac Defects
Gene Expression Manipulation
Morpholino Technology
RNA Injection
Forward Genetic Screens
Whole Mount Immunostaining
Expression Pattern
Targeted Protein
High Resolution Images
Cellular Structures
Subcellular Structures
Physical Location Of The Heart
Poor Penetration Of Antibodies
Staining Process
Microscope Slide
ImmEdge Pen
Fluorescence Signals
Article
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