JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
在哺乳动物细胞中细胞周期位置分析
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Analysis of Cell Cycle Position in Mammalian Cells
Please note that all translations are automatically generated.
Click here for the English version.
在哺乳动物细胞中细胞周期位置分析
DOI:
10.3791/3491-v
•
12:19 min
•
January 21, 2012
•
Matthew J. Cecchini
,
Mehdi Amiri
,
Frederick A. Dick
1
Department of Biochemistry, Schulich School of Medicine and Dentistry
,
University of Western Ontario
,
2
London Regional Cancer Program, Children’s Health Research Institute, and Department of Biochemistry, Schulich School of Medicine and Dentistry
,
University of Western Ontario
Chapters
00:05
Title
01:19
Labeling and Fixing of Cells
02:30
Denaturing and Staining of Brdu and DNA
04:11
Flow Cytometry and Data Analysis
06:10
Alternate Protocol for Cell Cycle Analysis in a Mixed Population of Cells
07:35
Cell Cycle Distribution Staining Can Be Used for a Variety of Experimental Purposes
11:47
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
确定的人口细胞的细胞周期的位置,或理解信号是如何影响扩散,可以很容易地通过使用此协议的流流式细胞仪测量。我们报告一个简单的实验方法,染色细胞和量化其在细胞周期中的位置。
Tags
Cell Cycle Position
Mammalian Cells
Cell Proliferation
Cell Cycle Phases
DNA Synthesis
Growth Regulation
Propidium Iodide
Flow Cytometry
Radioactive Thymidine
Bromodeoxyuridine
Fluorodeoxyuridine
BrdU Staining
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
长期使用宽视场显微镜的成像哺乳动物细胞
N -连接糖链的糖蛋白在哺乳动物细胞中的脉冲大通分析
在哺乳动物细胞中的DNA双链断裂(DSB)的维修分析
在哺乳动物细胞的RNAi筛选使命esiRNA
异源表达在哺乳动物细胞离子通道的突变和功能分析
在哺乳动物细胞通过显微注射mRNA的核出口动力学分析
哺乳动物链接组蛋白亚型表达分析
在哺乳动物细胞中的内质网定位的mRNA的可视化
诱导上皮和分析间质转化
中血红素的合成水平在哺乳动物细胞中测量
Read Article