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Biology
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RNAi筛选识别胚后的表型
C。线虫</em
JoVE Journal
Biology
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JoVE Journal
Biology
RNAi Screening to Identify Postembryonic Phenotypes in
C. elegans
Please note that all translations are automatically generated.
Click here for the English version.
RNAi筛选识别胚后的表型
C。线虫</em
DOI:
10.3791/3442-v
•
09:40 min
•
February 13, 2012
•
Katherine K. Beifuss
,
Tina L. Gumienny
1
Department of Molecular and Cellular Medicine
,
Texas A&M University System Health Science Center
Chapters
00:05
Title
01:24
Preparation of Bacteria with Library Clones
03:14
Preparation of Nematodes
06:51
Observation of Nematodes
08:55
Conclusion
Summary
Automatic Translation
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我们描述了致敏的方法来确定胚后调节蛋白表达和定位<em> C。线虫</em>使用RNAi技术为基础的基因组的屏幕和一个综合的功能，荧光标记的蛋白质基因的表达。
Tags
RNAi Screening
C. Elegans
Postembryonic Phenotypes
Model System
Genes
Gene Pathways
Functional Characterization
Protocol
RNAi Screen
Phenotype Assay
Fluorescent Product
Transgene
Genomic RNAi Library
CDNA Inserts
RNAi-sensitive Background
Hatched Animals
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