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Journal
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Immunology and Infection
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重组腺相关病毒载体的生产和滴定
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Production and Titering of Recombinant Adeno-associated Viral Vectors
Please note that all translations are automatically generated.
Click here for the English version.
重组腺相关病毒载体的生产和滴定
DOI:
10.3791/3348-v
•
08:35 min
•
November 27, 2011
•
Christina McClure*
1
,
Katy L. H. Cole*
1
,
Peer Wulff
,
Matthias Klugmann
,
Andrew J. Murray
3
1
School of Medical Sciences, College of Life Sciences and Medicine
,
University of Aberdeen
,
2
Translational Neuroscience Facility and Department of Physiology
,
School of Medical Sciences, University of New South Wales
,
3
Department of Biochemistry and Molecular Biophysics
,
Columbia University
Chapters
00:05
Title
01:15
Transfection of Viral Plasmids into Human Embryonic Kidney 293 (Hek293) Cells
02:53
Harvesting of rAAVs
04:19
Heparin Column Purification and Concentration of rAAVs
06:15
Titering of Viral Stocks
07:25
Representative Results Using rAAVs
08:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
重组腺相关病毒(rAAVs)载体正变得越来越有价值的<em>在体内</em在动物身上的实验。我们描述了如何rAAVs可以在实验室中产生,并如何将这些载体可以被滴定给一个准确的读数产生的传染性颗粒数量。
Tags
Recombinant Adeno-associated Viral Vectors
AAV
In Vivo Studies
Human Clinical Trials
Transduction Profile
Immune Response
Transgene Expression
Gene Delivery
RAAVs
Safety Profile
Chimeric RAAVs
AAV1
AAV2
Affinity Chromatography
Infectious Properties
Heparin Column Purification
Viral Titer
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