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Chapters
- 00:05Title
- 01:20Experimental Design and GoClone 3’UTR Reported Selection
- 02:36Co-transfection of 3′ UTR Reporters and microRNA
- 05:00Measurement of Luciferase Activity with LightSwitch Assay Reagents
- 05:48Luciferase Knockdown Assay: Analysis and Representative Results
- 06:54Conclusion
I microRNA (miRNA) sono importanti regolatori dell'espressione genica e hanno dimostrato di giocare un ruolo in numerosi processi biologici. Per capire meglio miRNA-UTR interazioni, abbiamo creato un genoma collezione di 3 giornalisti UTR luciferasi in coppia con un nuovo gene luciferasi e dosaggio reagenti, il sistema LightSwitch.
Tags
LightSwitch Luciferase Assay System3’UTR-reporter ConstructsMicroRNAs (miRNAs)Gene Expression RegulatorsComputational ToolsExpression AssaysProteomics AssaysChromatin-immunoprecipitation-based TechniquesMiRNA-3’UTR InteractionsFunctional EvidenceCell-based SystemGenome-wide Collection Of Human 3’UTR Luciferase ReportersSynthetic MiRNA Target Reporter ConstructsEndogenous 3’UTR Reporter ConstructsStandardized Experimental ProtocolsCo-transfectionMiRNA MimicHigh-throughput Analysis
