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球体的检测,以测量TGF -β诱导的入侵
JoVE Journal
Medicine
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JoVE Journal Medicine
Spheroid Assay to Measure TGF-β-induced Invasion
DOI:

09:18 min

November 16, 2011

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Chapters

  • 00:05Title
  • 01:05Preparation of Methocel Solution
  • 01:55Spheroid Culture
  • 03:00Preparation of Collagen and Spheroid Embedding
  • 07:04Representative Results
  • 08:46Conclusion

Summary

Automatic Translation

描述一个分析,定量分析转化生长因子(TGF)-β诱导的三维胶原凝胶侵袭。这个实验需要的细胞株MCF10A系列,代表不同阶段乳腺癌的发展优势。通过这种方法可以用于其他细胞系,并可能被用来调查其他潜在的激活或入侵抑制剂。

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