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Journal
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Immunology and Infection
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Epstein - Barr病毒生长转化的淋巴细胞系的建立
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
Please note that all translations are automatically generated.
Click here for the English version.
Epstein - Barr病毒生长转化的淋巴细胞系的建立
DOI:
10.3791/3321-v
•
06:38 min
•
November 08, 2011
•
Joyce Hui-Yuen
2
,
Shane McAllister
2
,
Siva Koganti
,
Erik Hill
,
Sumita Bhaduri-McIntosh
2,3,4
1
Stony Brook Children’s Hospital
,
State University of New York at Stony Brook
,
2
Department of Pediatrics
,
State University of New York at Stony Brook
,
3
Department of Molecular Genetics
,
State University of New York at Stony Brook
,
4
Department of Microbiology
,
State University of New York at Stony Brook
Chapters
01:35
Isolation of Peripheral Blood Mononuclear Cells (PBMC)
02:53
Infection with Epstein-Barr Virus (EBV)
03:47
Expansion and Cryopreservation of Lymphoblastoid Cell Lines (LCL)
04:46
Success of Outcome can be Predicted by Flow Cytometric Analysis of Cells
05:58
Conclusion
Summary
Automatic Translation
English (Original)
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我们描述了一种用于发电使用EB病毒转化的B细胞株的方法。我们也说明了一种新的分析,可以识别注定要进行改造,早在感染后3天的B细胞。
Tags
Epstein-Barr Virus
Growth-transformed Lymphoblastoid Cell Lines
EBV Infection
B Cell Proliferation
Immortalization
EBV Latency
Virus-driven B Cell Proliferation
Tumorigenesis
Immunologic Assays
Human Monoclonal Antibodies
Mitogens
Phytohemagglutinin
Lipopolysaccharide
Pokeweed Mitogen
Cyclosporin A
T Cell-mediated Killing
High Titer EBV
Immunosuppressive Agent
Peripheral Blood Mononuclear Cells
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