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表达GFP -肌动蛋白的内皮细胞的肌动蛋白细胞骨架的研究
JoVE Journal
Biology
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Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin
Please note that all translations are automatically generated.
Click here for the English version.
表达GFP -肌动蛋白的内皮细胞的肌动蛋白细胞骨架的研究
DOI:
10.3791/3187-v
•
08:37 min
•
November 18, 2011
•
Travis M. Doggett
,
Jerome W. Breslin
1
Department of Physiology
,
Louisiana State University Health Sciences Center
Chapters
00:05
Title
01:27
Transfection of HUVEC with GFP-Actin
03:25
Set up: Live-cell Imaging Chamber and Stage Heater
05:20
Live-cell Imaging Microscope Data Acquisition and Analysis
06:14
Kymograph Analysis of HUVEC
08:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
显微成像可以表达GFP -肌动蛋白的内皮细胞骨架结构的动态变化特征。这种方法与使用固定标本的技术,在之前的相同细胞的肌动蛋白细胞骨架的时空变化提供了详细的评估,期间和之后,各种生理,药理,或炎症刺激。
Tags
Actin Cytoskeleton
Endothelial Cells
GFP-Actin
Permeability
Adhesive Junctions
Junctional Integrity
Cortical Actin Belt
Actin Stress Fibers
Centripetal Tension
Inflammatory Mediators
Endothelial Permeability
Live-cell Imaging
Dynamic Nature Of Actin Structures
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