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Journal
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Immunology and Infection
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动态活细胞成像的宿主 - 病原体相互作用的研究使用的光学陷阱
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging
Please note that all translations are automatically generated.
Click here for the English version.
动态活细胞成像的宿主 - 病原体相互作用的研究使用的光学陷阱
DOI:
10.3791/3123-v
•
09:17 min
•
July 28, 2011
•
Jenny M. Tam
,
Carlos E. Castro
,
Robert J. W. Heath
,
Michael K. Mansour
,
Michael L. Cardenas
,
Ramnik J. Xavier
,
Matthew J. Lang
,
Jatin M. Vyas
1
Department of Medicine, Division of Infectious Diseases
,
Massachusetts General Hospital, Harvard Medical School
,
2
Department of Mechanical and Aerospace Engineering
,
The Ohio State University
,
3
Center for Computational and Integrative Biology
,
Massachusetts General Hospital, Harvard Medical School
,
4
Dept. of Chemical and Biomolecular Engineering
,
Vanderbilt University
Chapters
00:05
Title
01:34
Labeling of Pathogens with Fluorescent Dye
02:52
Preparation of RAW 264.7 Macrophages for Chamber Slides
04:04
Selection and Manipulation of a Pathogen with the Optical Trap
06:18
Representative Images of Pathogen and Immune Cell Interactions After Trapping-Initiated Pathogen:Cell Contact
08:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
一种方法是描述单独选择,操作和使用光学陷阱,再加上一个旋转的磁盘显微镜图像活病原体。光陷阱提供了空间和时间的控制生物体和地方他们相邻的宿主细胞。荧光显微镜捕捉动态,以最小的扰动细胞间的相互作用。
Tags
Optical Trap
Host-pathogen Interactions
Dynamic Live Cell Imaging
Immune System
Phagocytic Cell
Microbe
Intercellular Contact Events
Phagocytosis
Cell-cell Contact
Optical Trapping
Optical Tweezers
Biological Research
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