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结核分枝杆菌综合肉汤稀释药敏试验微孔板格式为一线和二线药物
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format
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结核分枝杆菌综合肉汤稀释药敏试验微孔板格式为一线和二线药物

DOI:
10.3791/3094-v

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12:39 min

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June 24, 2011

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, , ,
1Department of Laboratory Medicine and Pathology,Mayo Clinic

Chapters

  • 00:05Title
  • 01:47Experimental Preparation and Biosafety Procedures
  • 03:13Preparation of the Inoculum
  • 05:05Plating the Inoculum
  • 08:07Determining Antibiotic Resistance Manually
  • 09:12Determining Antibiotic Resistance Using the Vizion Platform
  • 10:10Determining Sample Purity and Performing Colony Counts
  • 10:57Representative Antimicrobial Susceptibility Assay
  • 12:00Conclusion

Summary

Automatic Translation

Automatic Translation

结核分枝杆菌药敏试验是具有挑战性的，但对病人的护理至关重要。这微孔板格式提供12种antimycobacterial药物进行测试，并提供最低抑菌浓度（MIC）值，这将有助于在适当的治疗。

Tags

Antimicrobial Susceptibility TestingMycobacterium Tuberculosis ComplexFirst Line DrugsSecond Line DrugsBroth DilutionMicrotiter Plate FormatAgar Method Of ProportionMacrobroth TestingBACTECVersaTREKBacT/ALERTAgar Proportion MethodResistance CalculationDrug-containing AgarDrug-free AgarMacrobroth MethodsCritical Drug ConcentrationsMYCOTBTuberculosis Treatment

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