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生物人类免疫缺陷小鼠的微血管网络
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Bioengineering Human Microvascular Networks in Immunodeficient Mice
DOI:

06:55 min

July 11, 2011

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Chapters

  • 00:05Title
  • 01:00Culture of Human Cord Blood-derived Endothelial Colony-forming Cells (ECFCs) and Bone-marrow Derived Mesenchymal Stem Cells (MSCs)
  • 02:51Resuspension of Cells in Collagen/Fibronectin/Fibrinogen Solution (Day 0)
  • 04:05Injection into Immunodeficient Nude Mouse (Day 0)
  • 04:51Harvesting the Implants (Day 7) and Evaluation
  • 05:50Cell Morphology
  • 06:38Conclusion

Summary

Automatic Translation

在这里,我们描述了一种方法来提供人脐血源性内皮细胞集落形成细胞(ECFCs)和骨髓来源的间质干细胞(MSCS),胶原蛋白/纤维连接蛋白凝胶中嵌入皮下到免疫缺陷小鼠,。这种细胞/凝胶相结合,产生一个人的血管网,用鼠标血管连接。

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