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Ex vivo l'imagerie de cellules T dans murin tranches avec des ganglions lymphatiques et la microscopie confocale Widefield
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Chapters
- 00:05Title
- 01:18Preparing Slices from Lymph Nodes
- 03:58Isolation and Labeling of T cells from Mouse Lymph Nodes
- 05:08Plating Labeled T cells onto Lymph Node Slices
- 05:45Preparation of Microscope and Lymph Node Slices for Imaging
- 06:27Imaging T cells with a Widefield Inverted Microscope
- 07:24Imaging T cells with a Confocal Upright Microscope
- 07:53Representative Images and Movies of T cell Accumulation, Recruitment, and Motility within Lymph Node Slices
- 09:17Conclusion
Ce protocole décrit une méthode pour imager des cellules T fluorescentes introduites dans les tranches de ganglions lymphatiques. La technique permet en temps réel des analyses de la migration des cellules T avec grand champ traditionnel fluorescence ou microscopie confocale.
Tags
Ex Vivo ImagingT CellsLymph Node SlicesWidefield MicroscopeConfocal MicroscopeMigration Of T CellsChemokinesTwo-photon MicroscopyQuantitative InformationInteractions With Other CellsSlice AssayFluorescently Labeled T CellsAcutely Prepared Lymph Node SliceReal-time ImagingNatural EnvironmentMechanisms Underlying T Cell Migration
