JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
HIV - 1衣壳大会通过低温电子显微镜的结构和迭代螺旋实时空间重构
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
Please note that all translations are automatically generated.
Click here for the English version.
HIV - 1衣壳大会通过低温电子显微镜的结构和迭代螺旋实时空间重构
DOI:
10.3791/3041-v
•
12:38 min
•
August 09, 2011
•
Xin Meng
,
Gongpu Zhao
,
Peijun Zhang
1
Department of Structural Biology
,
University of Pittsburgh School of Medicine
Chapters
00:05
Title
02:04
Frozen-hydrated Electron Microscopy (EM) Specimen Preparation
03:22
Cryo-EM of HIV-1 Capsid Protein Tubular Assemblies
05:26
Helical Indexing
07:13
Three-dimensional Reconstruction
10:42
Representative Results: HIV-1 Capsid Assemblies Structure Determination
12:16
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本文介绍了一种方法来获得一个三维(3D)螺旋组装使用低温电子显微镜的分子结构。在这个协议中,我们使用的HIV - 1衣壳集会,说明详细的三维重建过程,实现了由迭代螺旋空间重构方法的密度图。
Tags
HIV-1
Capsid Assemblies
Cryo-electron Microscopy
Iterative Helical Real-space Reconstruction
Macromolecular Protein Complexes
Image Processing
Single Particle Electron Microscopy
Two-dimensional Electron Crystallography
Helical Objects
IHRSR Method
Filamentous Forms
Actin Filaments
Microtubules
Amyloid Fibers
Tobacco Mosaic Viruses
Bacteria Flagella
3D Density Map
Flexible And Disordered Helical Assemblies
Video Article
Protocols
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
免疫相关动态三级的HIV - 1 V3环冠R2的序列结构特点的评估
从头
折叠
V3的HIV - 1取向的基因型推理使用人口为基础的测序
微波辅助植物病毒病的快速诊断用透射电子显微镜
使用基因型的方法预测HIV-1辅助用途(归经)的序列分析
在体外
对HIV - 1核心脱壳
新工具展开调节性T细胞的HIV-1感染的个体
评估的HIV-1感染的CD4的先天传感<sup> +</sup>性T细胞浆细胞样树突细胞使用<em>体外</em>共培养体系。
HIV-1的三聚体包膜糖蛋白的构象评估使用基于细胞的ELISA检测
从HIV-1阳性者的血浆分离外来体的
HIV-1 Gag的可视化绑定到巨人的单片囊泡(GUV)膜
Read Article