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Immunology and Infection
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从现场收集到的蚊子传染病毒Vero细胞培养法检测
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
Please note that all translations are automatically generated.
Click here for the English version.
从现场收集到的蚊子传染病毒Vero细胞培养法检测
DOI:
10.3791/2889-v
•
07:23 min
•
June 09, 2011
•
Philip M. Armstrong
,
Theodore G. Andreadis
,
Shannon L. Finan
,
John J. Shepard
,
Michael C. Thomas
1
Center for Vector Biology and Zoonotic Diseases, Department of Environmental Sciences
,
The Connecticut Agricultural Experiment Station
Chapters
00:05
Title
01:13
Mosquito Sorting and Collection
02:39
Homogenization of Mosquitoes
03:25
Preparation of Vero Cells
04:49
Inoculation of Vero cells with Mosquito Pool Homogenates
05:38
Screening Vero Cells for Virus Growth
06:40
Viruses Detected in Field-collected Mosquitoes by Vero Cell Culture Assay
07:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个方法来处理和屏幕现场收集到的蚊子的病毒Vero细胞培养法的多样性。通过采用这种技术的,我们已检测到4个分类家庭在康涅狄格州收集蚊子的9种不同的病毒。
Tags
Infectious Virus
Field-collected Mosquitoes
Vero Cell Culture Assay
West Nile Virus
Dengue Virus
Chickungunya Virus
Surveillance And Control Programs
Viral Infection
Trap Location
Species Grouping
Pooled Specimens
Buffered Saline
Mixer-mill
Vero Cell Cultures
Cytopathic Effect
Diagnostic Assays
Virus Isolation
Connecticut USA
Human Disease
New Records
Article
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